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gene clusters and analyzed their involvement in biofilm formation. The CupB and CupC programs determined do not show up to Perform a task in biofilm development beneath the problems tested. These observations suggest that several elements are available to P. aeruginosa

to aid its binding to numerous surfaces and for interbacterial adhesion also. The wide variety of such attachment mechanisms, which happen to be seemingly differentially regulated, might reflect the intricate requirements of the organism in the course of the colonization of extensively various environmental niches.

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type I pili are important for First attachment, and that the insufficient People constructions abolished the attachment on the floor, in contrast to flagella-defective strains.

) plus the attachment phenotype was analyzed as Formerly described. In contrast to PAN67B, neither PAB3B nor PAC3B was altered inside their ability to kind a biofilm in microtiter dishes.

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adhesins, related to the adhesins in other microorganisms (14). We confirmed that mutants devoid of the purposeful CupA are faulty while in the development of biofilm, inside a way which is impartial with the presence of form IV pili. Using the available sequence in the PAO1 genome, we discovered extra cup

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